Extended Data Fig. 5.: Influence of 3-HK administration on midgut barrier function. | Nature Microbiology

Extended Data Fig. 5.: Influence of 3-HK administration on midgut barrier function.

From: Anopheline mosquitoes are protected against parasite infection by tryptophan catabolism in gut microbiota

Extended Data Fig. 5.: Influence of 3-HK administration on midgut barrier function.

a, DHE staining of midguts in control (-) and 3-HK-treated (+) mosquitoes at 0 h prior to (left) and 24 h (right) post normal blood meal. Scale bars represent 100 μm. Images are representative of at least eight midguts. b, TUNEL staining of midgut from controls (-) and 3-HK-treated mosquitoes (+) at 0 h prior to (left) and 24 h (right) post normal blood meal. Scale bars represent 50 μm. Images are representative of at least four midguts. c, PH3 staining of midguts from controls (-) and 3-HK-treated (+) mosquitoes at 0 h (left) prior to and 24 h (right) post infectious blood meal. Scale bars represent 100 μm. d, Quantification of PH3-positive cells in the midguts at 0 h prior to infectious blood meal. Data are presented as mean ± s.e.m (n = 9). (e) Quantification of PH3-positive cells in the midguts from controls (n=4) and 3-HK-treated (n=5) mosquitoes at 24 h post infectious blood meal. Data are presented as mean ± s.e.m. Significance was determined by two-sided Student’s t-test.

Back to article page