Extended Data Fig. 4: Additional representative images from the arrayed screen and comparisons to the pooled results. | Nature Microbiology

Extended Data Fig. 4: Additional representative images from the arrayed screen and comparisons to the pooled results.

From: Screening the Toxoplasma kinome with high-throughput tagging identifies a regulator of invasion and egress

Extended Data Fig. 4: Additional representative images from the arrayed screen and comparisons to the pooled results.

a–c, Widefield microscopy of representative clones. Maximum intensity projections for IMC1-tdTomato and mNeonGreen-tagged targets are displayed for cultures treated with either IAA or vehicle for 24 hours. All images are displayed at the same scale. Localizations to puncta (a), the basal end (b), or peripheral structures (c) were assigned to a gene if half or more of single-integrated wells for that gene displayed consistent localizations. d, Representative confocal images of a sample of clones. mNeonGreen (green); IMC1-tdTomato (magenta). Images are maximum intensity projections. Genes are numbered based on the unique identifier from ToxoDB (for example, TGGT1_210830, labeled 210830). e, Comparison of relative gRNA abundances in the array compared to the pooled population that was subcloned. Spearman correlation coefficient = 0.77. f, Impact of the initial lytic cycles on gRNA abundance for genes with delayed or acute loss phenotypes in the HiT screen. The effect of the first lytic cycle from the HiT screen is plotted against the effect of the first or second lytic cycles for the genome-wide knockout screen (Sidik & Huet, et al. 2016). Genes are paired across their first and second lytic cycles within the genome-wide knockout screen.

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