Extended Data Fig. 2: Proline reductase gene clusters and in vitro phenotypes of Clostridium sporogenes mutants. | Nature Microbiology

Extended Data Fig. 2: Proline reductase gene clusters and in vitro phenotypes of Clostridium sporogenes mutants.

From: Clostridium sporogenes uses reductive Stickland metabolism in the gut to generate ATP and produce circulating metabolites

Extended Data Fig. 2

A) The two gene clusters for proline metabolism in C. sporogenes encode multiple copies of the prdA, prdB, prdC, and prdG genes. Red arrows designate mutants tested in this study for proline metabolism. Locus tag IDs for the two gene clusters are provided. B) Wild-type C. sporogenes and two ClosTron insertional mutants in prdR were cultured in defined medium containing 20 amino acids and 5-aminovalerate in the supernatant was quantified at 3, 6, 24, and 48 h by LC-MS. Numbers after the prdR name indicate positions in the gene where the group II intron was integrated. Experiments were performed in triplicate and data are reported as means ± standard deviations. C) Wild-type C. sporogenes and a prdF mutant were cultured in defined medium containing 20 amino acids and arginine and proline in the supernatant was quantified at 3, 6, 24, and 48 h by LC-MS. Data are plotted as means ± standard deviations from n = 3 experiments.

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