Extended Data Fig. 8: Pyroptosis in gut ILC3s was induced by S. Typhimurium, (Related to Fig. 5).
a and b, Analysis of Ki-67 and RORγt expression in ILC3s from LI-LPLs of uninfected and S. Typhimurium-infected WT mice on d.p.i 2. Representative FACS data of two independent experiments. Percentages of Ki-67+ cells in ILC3s (b). Compiled data from two independent experiments (n = 6 mice per group). c and d, Analysis of Aqua and IL-22 expression in LI ILC3s of uninfected and infected WT mice on d.p.i 2. Representative FACS data of two independent experiments (c). Percentages of Aqua+ cells in IL-22+ vs IL-22− ILC3s (d). Compiled data from two independent experiments (n = 5 and 7 mice for uninfected and infected groups, respectively). e, FACS analysis of active caspase-1 expression (Ac-YVAD-cmk, FLICA assay) in LI ILC3s of Casp1+/+ and Casp1−/− mice. Representative data of two independent experiments f, FACS analysis of active caspase-1 expression in LI ILC2s of uninfected and infected WT mice on d.p.i 2. Representative data of two independent experiments. g, FACS analysis of Aqua and Annexin V expression in LI ILC3s uninfected and infected with S. Typhimurium in vitro. Cells were pretreated with Ac-YVAD-cmk or Disulfiram as indicated. Representative data of two independent experiments. h, FACS analysis of IL-22 and IL-17 expression in MNK-3 cells, with or without P/I stimulation. Representative data of two independent experiments. i, Cell viability of MNK-3 cells infected with S. Typhimurium counted by FACS. Compiled data from three independent experiments (n = 6 independent wells). j, Western blot analysis to determine GSDMD in cell lysates of MNK-3 cells uninfected or infected with S. Typhimurium. Representative data of two independent experiments. k, Quantitative evaluation of mature IL-1β secreted in the culture supernatant of S. Typhimurium-infected MNK-3 cells using ELISA, with or without pretreatment of Ac-YVAD-cmk or Disulfiram. l, Quantitative evaluation of IL-22 secreted in the culture supernatant of S. Typhimurium-infected MNK-3 cells using ELISA, with or without pretreatment of Ac-YVAD-cmk or Disulfiram.
