Extended Data Fig. 7: Cryo-EM and single-particle analysis of OmpK36-TraN.
From: Mating pair stabilization mediates bacterial conjugation species specificity
a Overview of the image processing workflow done in cryoSPARC v3.2.0. 13,056 movies were selected based on estimated resolution, defocus, ice thickness, and full-frame motion. Accepted micrographs were subjected to automated particle picking ‘blob picking’ and 2x 2D classification to obtain template references. Template-based picking followed by 3x 2D classification was completed, resulting in 508, 537 particles. Particles were then used for Ab initio reconstruction; the best output reconstruction generated by Ab initio reconstruction was further refined using Non−uniform Refinement. The results of Non-uniform refinement yielded a reconstruction with an estimated resolution of 2.6 Å. b Coulomb potential density for the OmpK36 trimer (blue density) and TraN (green density). c Close up view of the density for the OmpK36 and TraN ‘tip’ side chains.
