Extended Data Fig. 6: Cholesterol availability in vitro and in vivo.
From: A biosynthetic pathway for the selective sulfonation of steroidal metabolites by human gut bacteria
a, B. thetaiotaomicron WT cultured in different types of media (BHI + , defined media, and ‘enhanced’ defined media) with and without lecithin (10 mg/L) exhibited lower levels of Ch-S production compared to BHI + media. Data are shown as mean ± SEM with one-way ANOVA with Dunnett’s correction, comparing to BHI + production, n = 3 biological replicates. b, The OD600 of cultures from a) was taken after 48 hour of incubation. No significant differences in growth levels were observed between pairs of cultures. Data are shown as mean ± SEM with Welch’s two tailed t-test comparing between pairs of cultures, n = 3 biological replicates. c, Levels of Ch quantified by GCMS in cecal contents of germ-free (n = 5) and SPF (n = 5) mice. Data are presented as mean ± SEM with Welch’s two-tailed t-test. d, Levels of Ch quantified by GCMS in fecal pellets of germ-free (n = 5) and SPF (n = 5) mice. Data are presented as mean ± SEM with Welch’s two-tailed t-test. e, Concentration of Ch found in human feces (n = 2 biological samples) in technical triplicate. Data are shown as mean ± SEM.
