Extended Data Fig. 7: CRISPR construct and genetic localization of the mutants.
From: A haem-sequestering plant peptide promotes iron uptake in symbiotic bacteria
a, Sequence of genetic locus of NCR247. Pale green is the 5’UTR region, dark yellow is the NCR247 coding region (signal sequence and coding region separated by an intron), Grey is part of the 3’UTR region. Location of gRNAs are marked in bold. The homozygous deleted region common in all the mutants is designated in red. b, Sequences of the gRNAs used in two different CRISPR constructs used. c, Location of deletions and substitution mutations in the 8 homozygous hairy roots obtained when compared to the wildtype sequence. Mutant 1-6 was obtained when the CRISPR construct 1 was used and Mutant 7-8 was obtained using CRISPR construct 2. d, Total iron content of the whole nodules isolated from wildtype and NCR247 knockdown roots as measured by ICP-MS analysis. 28 nodules from each type were collected and analysed in two technical replicates. In d, **P = 0.007 WT vs NCR247 knockdown; two tailed unpaired t test.
