Extended Data Fig. 9: NCR247 suitability for potential therapeutic applications.
From: A haem-sequestering plant peptide promotes iron uptake in symbiotic bacteria
a, Standard cytotoxicity assay (Methods) on HEK293 cell line indicates negligible hemolysis by L and D-NCR247. b, Standard hemolysis assay (Methods) on hRBC indicates negligible hemolysis by L and D-NCR247. In a and b, Triton X-100 was used as a positive lysis control and data was normalized to PBS blank. Data are presented as mean of three independent replicates ± s.d. c, d, Pull down of heme by biotinylated NCR247 from 0-day old (c)and 42-day old plasma(d). Oxalic acid assay was used to measure the total heme content of the pull-down. Data are presented as mean of three independent replicates (with three technical replicates for each) ± s.d. In a, b, **P = 0.0016, ***P = 0.0009 vs TritonX-100 treated sample and in d, and ****P < 0.0001 vs beads only; two-way analysis of variance (ANOVA) with multiple comparisons.
