Extended Data Fig. 7: RT-msDNA interactions in Ec86 complex. | Nature Microbiology

Extended Data Fig. 7: RT-msDNA interactions in Ec86 complex.

From: Cryo-EM structures of Escherichia coli Ec86 retron complexes reveal architecture and defence mechanism

Extended Data Fig. 7

Middle panel, overall structure of one Ec86 protomer. a, The X and Y regions in Ec86 RT. The X region (dodger blue) contains a long loop (between α4 and α5) and α5 and interacts with the RNA segment of DNA-RNA duplex and a short loop (between α5 and β3). The Y region (light sky blue) contains a long loop (between β6–α11) and α11. The conserved residues N105, A106, and H109 in X region and VTG (243-245) in Y region are close to the catalytic core YADD. b, Interaction between ssRb and the palm-finger domain. c, The interactions between ssDa region and palm domain. d, The interactions of ssDb with palm domain.

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