Extended Data Fig. 1: Purification of the Ec86 and effector-bound Ec86 complexes.
a, Purification of the Ec86 complex is indicated by a representative gel filtration chromatography, SDS-PAGE, and 12 % urea-PAGE. The peaks containing the target complex are illustrated by a black arrow and grey shadow. Fractions in lane 5 was used for cryo-EM sample preparation. b, Purification of the effector-bound Ec86 complex similar to the processes described in (a). Fractions in lane 6 was used for cryo-EM sample preparation. Lane 1, homemade DNA ladders. The msDNA (DNA-RNA hybrid) migrates slower than DNA. Images in (a) and (b) are representatives of three independent experiments.
