Extended Data Fig. 5: LC-MS analysis of amino acids in unspent and spent media.

LC-MS analysis of the unspent (n = 6 biological replicates) and spent SKL, SKN, and SKLN media (n = 3 biological replicates) was used to study amino acid consumption. Supernatants were collected at the (a) mid-exponential and (b) late exponential phases. Data represent mean ± SEM, analyzed by one-way ANOVA and Tukey’s HSD test. NS, not significant.