Extended Data Fig. 5: Recognition of crRNA by SbCas7-11. | Nature Microbiology

Extended Data Fig. 5: Recognition of crRNA by SbCas7-11.

From: Structure and function of a bacterial type III-E CRISPR–Cas7-11 complex

Extended Data Fig. 5

a, The recognition of crRNA by SbCas7-11. The finger and thumb regions are shown in ribbon representations. The other areas have been omitted for clarity. The 5-nt bracket sequence is indicated by arrows. b, Close-up view of the interactions between the crRNA repeat region and SbCas7-11. The crRNA-interacting residues of SbCas7-11 are shown as sticks. c, Close-up view of the interactions between the crRNA spacer region and SbCas7-11. d, In vitro target RNA cleavage assay of WT and mutant SbCas7-11 proteins. These gels are representative of three replicate experiments.

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