Extended Data Fig. 6: IPD may associate with crRNA and regulate target RNA cleavage.
From: Structure and function of a bacterial type III-E CRISPR–Cas7-11 complex
a, Cryo-EM density of SbCas7-11-crRNA complex with the focused refined IPD region. An atomic model predicted with AlphaFold2 was docked and refined with the density. An extra density (circled in magenta) was observed in IPD, potentially attributed to crRNA. b, Superimposition of the predicted IPD domain and CSD1 domain in the Upstream-of-N-ras (Unr) protein (PDB: 4QQB). c, In vitro target RNA cleavage assay shows that the IPD domain is dispensable for the target RNA cleavage activity of SbCas7-11. This gel is a representative of three replicate experiments. Del-IPD-1: The IPD region (aa 1032-1387) was replaced with a GGGS linker in SbCas7-11. Del-IPD-2: The IPD region (aa 1038-1383) was replaced with a GSAS linker in SbCas7-11.
