Extended Data Fig. 7: Catalytic sites of Cas7.2 and Cas7.3 for target RNA cleavage.
From: Structure and function of a bacterial type III-E CRISPR–Cas7-11 complex
a, Structural comparisons of Cas7.2 and Cas7.3 domains in SbCas7-11 with Csm3 protein (white, PDB: 6NUE). b, Close-up view of the catalytic sites. The catalytic D33 residues in Csm3 are indicated by arrows. Corresponding residues and those in close proximity of SbCas7-11 are marked. c, In vitro target RNA cleavage with SbCas7-11 variants bearing mutations in Cas11 and Cas7.2 domains. These gels are representative of three replicate experiments. d, Representative target RNA cleavage assay for SbCas7-11 WT and mutants. Time courses represent 5, 15, and 30 min. This gel is a representative of three replicate experiments. e, In vitro target RNA cleavage with DiCas7-11 variants bearing mutations in Cas7.2 domain. The gel is a representative of three replicate experiments. f, Superimposition of the SbCas7-11-crRNA binary complex (white) and the SbCas7-11-crRNA-target RNA ternary complex. The Cas11 domain rotates outward upon binding of target RNA.
