Extended Data Fig. 8: Structural analysis of Csx29 and SbCas7-11 in different complexes.
From: Structure and function of a bacterial type III-E CRISPR–Cas7-11 complex
a, Superimposition of the PS1 and PS2 domains in Csx29. The PS1 subdomain is roughly similar to PS2 despite the lack of the catalytic dyad. b, In vitro pull-down of Csx29 WT and mutants by MBP-tagged SbCas7-11 proteins. The Csx29 bands are indicated by red stars. This gel is a representative of three replicate experiments. c, Superimposition of the SbCas7-11-crRNA complex (white) and the SbCas7-11-crRNA-Csx29 complex. d, Superimposition of the SbCas7-11-crRNA-taget RNA complex (ruby) and SbCas7-11-crRNA-Csx29 complex.
