Extended Data Fig. 8: Co-localisation of PfCSS, PfPTRAMP and CyRPA in late schizonts of P. falciparum by immunofluorescence and super resolution microscopy.
From: PCRCR complex is essential for invasion of human erythrocytes by Plasmodium falciparum
a. Localisation of PfCSS-HA (green) detected with anti-HA antibodies compared to PfPTRAMP detected using mAb 3D8 (purple). b. Localisation of PfCSS-HA (green) detected with anti-HA antibodies compared to CyRPA detected using mAb 8B9 (purple). c. Localisation of PfPTRAMP (green) detected with anti-HA antibodies compared to CyRPA detected using mAb 8B9 (purple). Shown for all panels is merge+DAPI where nuclei have been stained and PfCSS, PfPTRAMP and DAPI channels have been merged. Fourth panels on the right includes merge of all as well as DIC (differential interference contrast). The far-right panels indicate co-localisation of each protein across the broken white line of the merge+DAPI panels. The scale bar in merge + DIC panels is 2 μM and is relevant for all panels.
