Extended Data Fig. 7: Extending the pre-integration stage of infection with raltegravir increases the percentage of latently infected cells.
From: Epigenetic silencing by the SMC5/6 complex mediates HIV-1 latency
WT or ΔSMC5 CEM-SS cells were infected with IN + NL-GFPΔEnv in the presence and absence of the integrase inhibitor raltegravir. These cells were infected at a multiplicity of infection (MOI) such that the GFP + cells in both the +ral and -ral were roughly identical when sorted at 5dpi, as indicated in panel A. At 2dpi, the cells were washed to remove raltegravir and replated to allow integration to occur. GFP- cells were isolated by FACS at 5dpi and cultured for another 6 days (11dpi) where they were treated with diluent (DMSO), TAK-981, PMA, or TNF-α. The cells were then analyzed by flow cytometry at 12dpi for GFP expression. (a) Representative GFP expression profiles from a single experiment. (b) Compilation of 3 independent biological replicates, showing the means and with SD indicated. Data analyzed by 2-way ANOVA, Tukey’s test (****p < 0.0001).
