Extended Data Fig. 8: The SMC5/6 promotes HIV-1 latency.
From: Epigenetic silencing by the SMC5/6 complex mediates HIV-1 latency
(a) In WT HIV-1 infected primary CD4 + T cells, unintegrated HIV-1 DNA peaks at 2dpi and declines to background levels by 7dpi, as measured by qPCR analysis of 2LTR circular viral DNA. These data represent the average of five biological replicates using CD4 + T cells obtained from five separate blood donors, with SD indicated. Data were normalized to the signal detected at 2dpi, which was set at 1.0. (b) Statistical analysis of the indicated GFP/mCherry subpopulations in cells infected with the dual color HIV-1 indicator virus and treated with 150 nM TAK-981 at 6dpi vs diluent (DMSO) at 0dpi. Infected cells were harvested at 7dpi and analyzed by FACS. Statistics calculated with a 2-tailed ratio paired t-test.
