Extended Data Fig. 1: CEM-SS Cas9 cells infected with IN- HIV-1 show increased viral gene expression when any component of the SMC5/6 complex is knocked down.
From: Epigenetic silencing by the SMC5/6 complex mediates HIV-1 latency
(a-b) Polyclonal populations of CEM-SS Cas9 cells transduced with 2 different sgRNAs specific for the indicated genes and then infected with (A) IN- NL-GFPΔEnv, and (B) IN- NL-NLucΔEnv reporter virus. %GFP + cells in A were determined by flow cytometry at 2dpi. NLuc expression in B was normalized to CEM-SS Cas9 cells transduced with a scrambled sgRNA, which was set to 1.0. Data shown in panel B represent the average of three biological replicates with SD indicated.
