Extended Data Fig. 4: Histone H3 and NSMCE2 binding to HIV-1 DNA.
From: Epigenetic silencing by the SMC5/6 complex mediates HIV-1 latency
The indicated cell lines were infected with IN + and IN- NL-GFP and subjected to ChIP–qPCR at 2 dpi to quantify the levels of the activating histone modifications H3Ac and H3K4me3 and repressive modifications H3K9me3 and H3K27me3 bound to viral LTR DNA. (a) H3Ac and (b) H3K4me3 (c) H3K9me3, (d) total histone H3, and (e) H3K27me3. Average of three biological replicates with SD indicated. Data was analyzed with 2-way ANOVA, Sidak’s test (****p < 0.0001; IN- H3K4me3 ΔSMC5: ***p = 0.0009; IN- H3K4me3 ΔSLF2: ***p = 0.0007).
