Extended Data Fig. 6: Siderophores from diet and mycobiota promote Salmonella gut colonization.
From: Mycobiota and diet-derived fungal xenosiderophores promote Salmonella gastrointestinal colonization
(a) mRNA expression of innate immune genes extracted from cecal tissue 96 h post infection with WT Salmonella or iroN fepA Salmonella. Expression is normalized to housekeeping gene Actb (Tnf, Ifng, Il17a, Il22 n = 8; Cxcl1 WT n = 7, iroN fepA n = 8). (b) Colonization measured as CFU/mg of Salmonella strains iroN fepA and iroN fepA fhuA in fecal samples of SPF mice collected at different time points post infection [24 h (n = 8), 48 h (n = 8), 72 h (n = 5), 96 h (n = 8)]. (c) Dissemination measured as CFU/mg of Salmonella strains iroN fepA and iroN fepA fhuA in peripheral organs of SPF mice collected at 96 h post infection [liver, spleen (n = 7), Peyer’s patches (PP) and mesenteric lymph nodes (MLN) (n = 8)]. (d) Competitive index of Salmonella strain iroN fepA over iroN fepA fhuA in organ homogenate liver, spleen (n = 7), Peyer’s patches (PP) and mesenteric lymph nodes (MLN) (n = 8) at 96 h. (e) Colonization measured as CFU/mg of Salmonella strains iroN fepA and iroN fepA fhuA fhuE in fecal samples of ASF mice collected at different time points post infection [24 h (chow n = 19, purified n = 20), 48 h (chow n = 19, purified n = 20), 72 h (chow = 15, purified n = 16), 96 h (chow n = 14, purified n = 16)]. (f) Competitive index (CI) of Salmonella strain iroN fepA over iroN fepA fhuA fhuE in fecal samples of ASF mice at 24 h, 48 h, 72 h, and 96 h. Each line represents CI development in one mouse (shown are mice with samples at more than 2 time points; chow n = 15, purified n = 16). (g) Slope of CI development in (f) (mice with samples at all time points; chow n = 14, purified n = 16). (h) CI of Salmonella strain iroN fepA over iroN fepA fhuA fhuE in fecal samples of ASF mice at 96 h compared to 24 h (mice with samples at both time points; chow n = 14, purified n = 16). (i) Colonization measured as CFU/mg of Salmonella strains iroN fepA and iroN fepA fhuA fhuE in fecal samples of ASF mice collected at 96 h post infection (chow n = 5, purified n = 6, purified + fungi n = 7). Data are depicted as geometric mean +/− geometric SD (a-c, e, i), box plot from 25th to 75th percentile, median, minimum and maximum (d), or median with 95% confidence interval (g, h). Statistics: For panel (a), (d), (g), and (h) one sample t test compared to no competitive advantage (CI = 1) was used. For panel (g) and (h) unpaired Mann-Whitney test was used. Numerical p values are indicated within the figure.
