Extended Data Fig. 1: Purified fungal siderophores promote Salmonella growth.
From: Mycobiota and diet-derived fungal xenosiderophores promote Salmonella gastrointestinal colonization
(a) Growth of Salmonella strains WT (n = 5), iroN (DMEM n = 5, DMEM + Lcn2 n = 6), iroN fhuA (DMEM n = 3, DMEM + Lcn2 n = 4), iroN fhuE (n = 3) at different time points (2, 5, 8 h) in DMEM/F12 + 10% FBS or DMEM/F12 + 10% FBS + 100 ng/ml lipocalin-2. (b) Growth of Salmonella strains WT, iroN, iroN fhuA or iroN fhuE in DMEM/F12 + 10% FBS + 100 nM coprogen (n = 3) or DMEM/F12 + 10% FBS + 100 nM ferrichrome (n = 3). (c) Growth of Salmonella strains WT, iroN fepA and iroN fepA fhuA fhuE in LB. Time points were taken at 2, 5, 8 and 24 h (n = 3). (d) Growth of Salmonella strains WT and fhuA fhuE at 1 h, 3 h, 5 h, 7 h post inoculation in co-culture in minimal medium (n = 3), minimal medium + 100 nM ferrichrome (n = 3), minimal medium + 100 ng/ml deferoxamine (n = 3). Statistics: 2way ANOVA with Tukey’s multiple comparisons test. Data represent geometric mean + /- geometric SD. Numerical p values are indicated within the figure.
