Extended Data Fig. 6: Flow cytometry gating strategy for IgG plasma binding analysis. | Nature Microbiology

Extended Data Fig. 6: Flow cytometry gating strategy for IgG plasma binding analysis.

From: Divergent SARS-CoV-2 variant emerges in white-tailed deer with deer-to-human transmission

Extended Data Fig. 6

HEK 293 T cells were identified in forward scatter (FSC) and side scatter (SSC). Single cells (Singlets) were selected for analysis using FSC-A and FSC-H. Cells transfected with SARS-CoV-2 spike protein plasmid were gated (GFP+), negative and auto-fluorescent cells were excluded. Median fluorescence intensity for IgG (AlexaFluor 647-A) was analyzed in GFP + cells. Data was analyzed using FlowJo software.

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