Fig. 3: Gene-specific sweeps involving pilin biogenesis genes in ‘Ca. C. stinkeris’.

a, SNPs were called in ‘Ca. C. stinkeris’ after competitively mapping of metagenomic reads to the reference genomes from both populations. The percentage of detected SNPs was calculated in 1,000 bp windows and plotted. No SNPs were observed in a region spanning over 7 kB, the largest SNP-free region. b, The region identified in a was aligned between ‘Ca. C. stinkeris’ NA2 and ‘Ca. C. noahi’ NE3. The region included two genes annotated to be involved in pilin biogenesis, pilO and mshL. The pilO gene has been interrupted by a stop codon. Alignment identity of the region was calculated in 1,000 bp windows and plotted as a moving average.