Extended Data Fig. 1: Relative activity of MAGs in different metatranscriptomic datasets.

Data from two transcriptomic studies13,83 were mapped against the rumen epithelial MAGs. Reads mapping to predicted ORFs in the MAGs were counted and the reads mapped per kilobase (kB) were plotted for each treatment group. Mann et al.13 assessed the impact of sub-acute ruminal acidosis (SARA) on dairy cattle in Austria (n = 6), while Tan et al.83 compared beef cattle with low and high relative feed intake (RFI) in Canada (n = 16). Box plots show the median with hinges that correspond to the 25th and 75th percentiles. The whiskers extend from the hinge to the largest and smallest value no further than 1.5 multiplied by the inter-quartile range. Individual data points are overlaid.