Fig. 1: Study design, CD8+ T lymphocyte depletion and SIV plasma viral load kinetics.
From: CD8+ lymphocytes do not impact SIV reservoir establishment under ART
a, Study design. b, CD8+ T-cell kinetics in peripheral blood after depletion. c,d, Longitudinal flow cytometry analysis of Ki-67 (c) or PD-1 (d) expression in bulk CD8+ T cells after CD8+ T-cell depletion in the Pre-infection depletion group (n = 8 macaques, magenta line), Pre-ART group (n = 8 macaques, teal line) and in the control group (n = 5 macaques, black line). e, SIV plasma viral load in the first 120 d p.i. in the three experimental groups (n = 21 macaques). Limit of detection is 60 copies of SIV RNA per ml of plasma (horizontal dotted line). f, Plasma viral load comparison among the 3 groups at days 14 and 21 (left side) and days 28 and 35 (right side). g, Viral load 7 d decline rate. In a–e: blue arrow indicates SIVmac239M infection; red and orange arrows indicate the administration of 50 mg kg−1 MT807R1 before infection or ART initiation, respectively. Grey box represents time on ART. In b–e: data are mean ± s.e.m. Kruskal Wallis test was used to compare the values between the groups. In b–g: magenta squares/lines represent the Pre-Infection group (n = 8 macaques), teal triangles/lines represent the Pre-ART group (n = 8 macaques), black circles/lines represent the control group (n = 5 macaques). In f and g: the horizontal bar in each group represents the mean. A two-sided Welch’s t-test was used to compare values between the groups. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.
