Extended Data Fig. 6: In vitro Assembly of OmcZ nanowires from native OmcZ50.
From: Structure of Geobacter cytochrome OmcZ identifies mechanism of nanowire assembly and conductivity
a, TEM image of native G. sulfurreducens OmcZ50 showing lack of nanowire assembly. b, Western immunoblot using OmcZ antibody showing OzpA digesting OmcZ50 into OmcZ30. c, Digested OmcZ30. self-assembles into nanowires in vitro. d, SDS-PAGE gel of purified OzpA showing three major bands: premature protein, mature protein, and the cleaved inhibitor domain (I9). e, Power spectrum of the reconstituted OmcZ nanowires shows the same helical parameters as cell-produced OmcZ nanowires. f, 2D average of reconstituted OmcZ nanowires. g, CD spectra of reconstituted and native OmcZ nanowires is similar. Immuno-gold labeling of reconstituted OmcZ nanowires using h, No-primary antibody, i, antibody for OmcZ30 and j, antibody for OmcZ50. Scale bars, a, 100 nm. c, 50 nm. c, 2 nm. h, 100 nm, i, 50 nm, j, 100 nm.
