Extended Data Fig. 4: Functional metagenomic plasmid library delivery by gp17V544G displaying T7 bacteriophage particles into Shigella sonnei HNCMB 25021.
a, Functional metagenomic plasmid library delivery is as efficient by T7 gp17V544G bacteriophage particles into Shigella sonnei HNCMB 25021 as by electroporation into E. coli BW25113 (P = 0.19769, two-sample one-sided t-test, n = 3 biologically independent experiments. Centre and error bars represent mean and standard error.) Data available in Supplementary Table 2. b,c, Delivered metagenomic DNA fragment lengths and diversities, respectively, determined by using long-read deep sequencing right after electroporation and transduction (Methods). Dashed lines represent the average size of the DNA fragments. Shannon alpha diversity indices (H) were calculated based on the frequency of fragments with identical sequences in the libraries (n = 276899, n = 162107, for E. coli and S. sonnei, respectively). Data available in Supplementary Table 3.
