Extended Data Fig. 5: Nutrient downshifts of ∆prkC cells, and scaling of cellular dimensions under growth accelerated conditions.
From: Growth rate is modulated by monitoring cell wall precursors in Bacillus subtilis
a, Nutrient downshifts of WT (PY79) and ΔprkC (bYS542). Cells were assayed as they were shifted from growth in CH to growth in S750 glycerol. b, c, Cell width decreases (b), and cell length increases (c) with increasing growth rate in the growth accelerated mutants. WT (bME07), RodZ(S85E) (bSKH389), overexpressing murAA (bSKH55) with 30 mM xylose, or ectopically overexpressing prkC (bSKH387) with 30 mM xylose. Unless otherwise indicated, all strains were assayed in S750 glycerol. d, Cell volume remains constant in growth accelerated mutants- Volume for each cell is calculated as (π/6) D2+ (π/4) D2 (L-D), where D is the cell width and L is the cell length. e, The interdivision time of cells decreases as the accelerated cells grow faster – Interdivision time was measured by measuring the time between Z ring constrictions in mNeonGreen-pbp2B in S750 glycerol: WT cells (bME07), cells containing RodZ(S85E) (bSKH389), cells overexpressing murAA (bSKH55) with 30 mM xylose, or cells ectopically overexpressing prkC (bSKH387) with 30 mM xylose. Dashed lines represent the mean of WT values. All constructs are at the native locus unless otherwise indicated. N values are available in Supplementary Table 2. Error bars and envelopes represent standard deviation.
