Fig. 2: Increased Mtb replication in ATG7-deficient iPSDM.
a,c,e, Snapshot of live ATG7+/+ and ATG7−/− iPSDM infected with Mtb WT (a), ΔesxBA (c) and ΔcpsA (e) at 96 h. Nuclear staining (blue) and Mtb-E2-Crimson (red). Scale bars, 50 µm. b,d,f, High content quantitative analysis of Mtb replication after infection of ATG7+/+ or ATG7−/− iPSDM with Mtb WT (b), ΔesxBA (d) and ΔcpsA (f). Mtb area per cell was calculated as fold change, relative to 2 h post infection. Data representative from one out of two independent experiments (n = 3 independent wells). Results are shown as mean ± s.e.m. An unpaired two-tailed t-test was used for comparisons **P < 0.002; NS, non-significant. g, Representative images of Blue/Green (Live/Dead)-stained ATG7+/+ and ATG7−/− iPSDM uninfected (CTRL) or infected with Mtb WT for 96 h. Nuclear staining (blue) and dead nuclear staining (green). Scale bars, 50 µm. h, Quantitative analysis of the percentage of NucGreen-positive cells in each condition. Data representative from one out of two independent experiments (n = 3 independent wells). Results are shown as mean ± s.e.m. One-way ANOVA followed with Šídák’s multiple comparison test ***P < 0.001, *P < 0.033; NS, non-significant.
