Extended Data Fig. 3: Mtb phagocytosis and cell numbers after infection of ATG7−/− iPSDM. | Nature Microbiology

Extended Data Fig. 3: Mtb phagocytosis and cell numbers after infection of ATG7−/− iPSDM.

From: ATG7 and ATG14 restrict cytosolic and phagosomal Mycobacterium tuberculosis replication in human macrophages

Extended Data Fig. 3

a, Left graph shows the quantitative analysis of Mtb WT or DesxBA area in ATG7−/− or ATG7+/+ iPSDMs 2 h post infection. Right graph shows the percentage of infected cells at 2, 24, 48, 72 and 96 h post infection from the same biological replicate. Data representative from one out of two independent experiments (n = 3 independent wells). Results are shown as mean ± SEM. (b) The graph on the left represents the quantification of Mtb WT or DcpsA area in ATG7−/− or ATG7+/+ iPSDMs 2 h post infection. Right graph shows the percentage of infected cells at 2, 24, 48,72 and 96 h post infection from the same biological replicate. Data representative from one out of two independent experiments (n = 3 independent wells). Results are shown as mean ± SEM. (c) Western blot analysis of ATG7, p62 and LC3B levels in ATG7+/+ and ATG7−/− iPSDMs at 2 h, 24 h or 48 h of infection with Mtb WT, ΔesxBA or ΔcpsA. Representative Western blots from two independent biological replicates (n = 2).

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