Extended Data Fig. 5: Pearson’s correlations of quality-controlled reads from each sequencing library suggest that biological triplicates are highly similar, and all should be included in subsequent differential expression analysis.

As anticipated, lignocellulosic substrates induced more similar transcriptional profiles to one another than to purified cellulose or cellobiose, and grass (sorghum and switchgrass) lignocellulose treatments were more like one another than to poplar. Libraries are ordered as groups of replicates. Values in cells are Pearson’s correlation coefficients. Purple borders around replicate comparisons indicate acceptable correlation for continued analysis. Each identifier represents a single replicate. Samples correspond to the following identifiers: GTHNB = cellobiose replicate 1, GTHNC = cellobiose replicate 2, GTHNG = cellobiose replicate 3, GTHNH = purified cellulose replicate 1, GTHNN = purified cellulose replicate 2, GTHNO = purified cellulose replicate 3, GTHNP = switchgrass replicate 1, GTHNS = switchgrass replicate 2, GTHNT = switchgrass replicate 3, GTHNU = sorghum replicate 1, GTHNW = sorghum replicate 2, GTHNX = sorghum replicate 3, GTHNY = poplar replicate 1, GTHNZ = poplar replicate 2, and GTHOA = poplar replicate 3.