Fig. 3: 2D-HSQC-NMR data show that anaerobic fungi deconstruct aromatic and aliphatic regions in various lignins and remove lignin pendent groups.

a, Sorghum uninoculated control. b, Sorghum after N. californiae growth. c, Sorghum after A. robustus growth. d, Switchgrass uninoculated control. e, Switchgrass after N. californiae growth. f, Switchgrass after A. robustus growth. g, Poplar uninoculated control. h, Poplar after N. californiae growth. i, Poplar after A. robustus growth. Inset values provide relative comparisons of the lignin components determined from contour volume integrals where S + G = 100% (right insets) or A + B = 100% (left insets). Lignin H units are below the detectable limit, as are resinol and dibenzodioxocin structures. The pCA and pB pendent ester fractions are calculated on a ‘core-lignin’ basis meaning the integral for the ester divided by S + G (pendent ester/S + G = %). Legends for monomeric lignin subunits, pendent esters and units with their characteristic inter-unit linkages are colour-coded to match their signals in the spectra.