Fig. 4: netB toxin in C. perfringens is preferentially expressed by a subpopulation of cells and can be downregulated by the addition of acetate.
From: Probe-based bacterial single-cell RNA sequencing predicts toxin regulation
a, Merged UMAP of C. perfringens grown in parallel to stationary phase in BHI with and without acetate (4 mM). Far left: four populations identified after graph-based clustering. Middle left: merged UMAP of C. perfringens with shading representing normalized netB toxin expression per cell. Middle right: merged UMAP in which cells grown without acetate supplementation are highlighted in red. Far right: merged UMAP in which cells grown with acetate supplementation (4 mM) are highlighted in blue. b, Left: violin plot of normalized netB expression in all cells grown with or without acetate. P value < 0.01 by two-sided t-test. Right: violin plot of normalized netB expression in cells within cluster 0 grown with or without acetate. P value < 0.01 by two-sided t-test. c, Percentage of cells within each cluster for each condition (± acetate). d, Left: volcano plot of DGE of cells grown with versus without acetate supplementation. Counts normalized using DeSeq2 median of ratios method (ref. 45) and compared by two-sided t-test. Adjusted P values corrected by the method of Benjamini and Hochberg. Right: subset of volcano plot, focusing on genes downregulated by the addition of acetate with netB highlighted.
