Extended Data Fig. 10: Illustrative images and statistics of PI and CellEvent staining of macrophages infected with M. tuberculosis.
From: Autophagy restricts Mycobacterium tuberculosis during acute infection in mice
a, Illustrative images of experiment quantified in Fig. 6a, b. Autophagy-deficient macrophages were infected with M. tuberculosis at a MOI of 1. Propidium iodide and CellEvent Caspase-3/7 reagent were added to the media at the beginning of the infection. Cells were imaged every 30 minutes over a period of 8 days. Scale bar denotes 100 µm. b, Same data as in Fig. 6a, b but with selected timepoints presented as an individual graphs. Results are the means ± SD with each condition tested in technical triplicates. For statistical analysis, number of CellEvent (b) and propodium iodide (c) positive macrophages in cre-positive conditions were compared to the respective LysMwt/wt control. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001 by two-sided t-test.
