Extended Data Fig. 3: Additional in vivo data on autophagy-mutant mice infected with M. tuberculosis.
From: Autophagy restricts Mycobacterium tuberculosis during acute infection in mice
Mice (n = 10-12 per group, except for LysMcre+/+ for which n = 5) were infected with approximately 100 colony-forming units (c.f.u.) of M. tuberculosis and tracked for weight change (a-c). Data is presented as means ± SD. Mice that lost 15% body weight were sacrificed are counted at −15% for subsequent time points. d-e, CFU counts from the spleens of mice (n = 2-6 per group) infected with approximately 100 c.f.u of M. tuberculosis. Spleens were harvested at 21 days postinfection (d) or 70 days postinfection (e). Data is presented as means ± SD. f-g, Proportion of immune cell populations in the lungs of Atg7 (f) and Atg16L1 (g) mutant mice infected for 70 days with M. tuberculosis. Results are the means ± SD from 2–7 mice per condition. Nt = Neutrophils, Tc = T cells, Bc = B cells, Mac = Macrophages, DC = Dendritic cells, Mono = Ly6Chi inflammatory monocytes. For statistical analysis for CFU and histology experiments, LysMcre+/- and LysMcre+/+ conditions were compared to the respective LysMwt/wt control. * P ≤ 0.05, ** P ≤ 0.01 by two-sided Mann–Whitney test.
