Extended Data Fig. 5: BFD2 deletion in a conditional BFD1 strain.
From: A positive feedback loop controls Toxoplasma chronic differentiation
a, Schematic of ligand-inducible BFD1. The DD-BFD1-TY strain was constructed previously23 by integration at the HXGPRT locus in the Δbfd1 genetic background. A heterologous promoter (pTUB1) drives expression of the transgene but DD-BFD1-TY protein is only stabilized following treatment with Shield-1. b, Validation of BFD2 knockout in DD-BFD1-TY. Selected clones were screened using the same strategy described in Extended Data Fig. 3, verifying both loss of endogenous BFD2 and replacement with the fluorescence cassette. The gel (bottom) shows PCRs performed on gDNA from both the parental (DD-BFD1-TY) and DD-BFD1-TYΔbfd2 strains. The reverse primers used are listed over the respective well, with the expected product sizes indicated in the diagram (top).
