Extended Data Fig. 1: Genotyping of conditional depletion strains.
From: A positive feedback loop controls Toxoplasma chronic differentiation
a, Clones were screened to verify integration of mNG–mAID downstream of the targeted coding sequence (CDS) and the reciprocal loss of the untagged allele. The diagram (top) shows the binding sites for primers listed in the table (bottom), with regions used to direct construct integration (that is, H1 and H2; Fig. 1b) in dark grey. In each case a common gene-specific forward primer (P11–P16) was paired with reverse primers to either mNG (P17) or the respective endogenous 3′ UTR (P18–P23) downstream of the integration site. The expected PCR product size is given for each template and primer combination. Refer to Supplementary Table 9 for a complete list of the primer sequences. b, PCRs were performed on ME49/TIR1 gDNA (parental) as a control in addition to the respective tagged strain. Lanes are labelled with the reverse primer and gDNA template used in each reaction. For positive clones, bands were extracted and subjected to Sanger sequencing to verify in-frame integration of the tag.
