Extended Data Fig. 1: Gating strategy for Luminex-based and Fc effector function assays. | Nature Microbiology

Extended Data Fig. 1: Gating strategy for Luminex-based and Fc effector function assays.

From: Fc-γR-dependent antibody effector functions are required for vaccine-mediated protection against antigen-shifted variants of SARS-CoV-2

Extended Data Fig. 1

(a) Gating for Luminex-bead based antibody binding to spike-coated beads. (b) Gating for ADNP assay showing CD66+ neutrophils with opsinophagocytosed beads. (c) Gating for ADCP assay showing THP-1 monocytes and opsinophagocytosed beads. (d) Gating for ADCD assay showing complement deposition on spike and antibody coated beads. (e) Gating for NK cell activation assay showing CD107a expression.

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