Extended Data Fig. 2: T cell responses in mRNA-1273 vaccinated wild-type and FcγR KO mice.
(a) Representative flow cytometry plots show gating scheme for quantification of spike-specific CD4+ and CD8+ T cell responses in the spleen of wild-type, FcγR III KO and FcγR I/III/IV KO mice at day 10 after boosting with control or mRNA-1273 vaccines. (b-c) At day 10 after boosting, the spleen of wild-type and FcγR KO mice were harvested and T cell responses were measured after spike peptide re-stimulation. Splenocytes were incubated overnight with class I MHC (b) or class II MHC (c) immunodominant spike peptides, and the percentages and numbers of IFNγ and TNFα positive CD8+ (b) or CD4+ (c) T cells were quantified by intracellular staining and flow cytometry. Data are pooled from two experiments (in order left to right n = 10, 10, 8, 10, 10, 9 (b-c)). Comparisons were made between groups that received the mRNA 1273 vaccine (one-way ANOVA with Tukey’s post-test; all comparisons were not significant; column height indicates mean values).
