Extended Data Fig. 5: Construction and identification of the UvLAO2 mutation in U. virens.
From: Phyllosphere microbiome induces host metabolic defence against rice false-smut disease
a, The gene knock-out strategy of UvLAO2 in U. virens Genome. PCR primers used for verification were marked with small arrows. Hph, Hygromycin B phosphotransferase gene. Scale bar = 500 bp. b,c, Identification of UvLAO2 mutant (ΔUvlao2) by PCR assays using two primer pairs (including LAO2-F/R and LAO2up + hph-F/R). The LAO2-F/R-amplified product were detected in the genome of wild type (WT) of U. virens, but it was absent in ΔUvlao2 (b); The LAO2up + hph-F/R-amplified product was not detected in the genome of WT of U. virens, but detectable in ΔUvlao2 (c). d, DNA mutation of UvLAO2 gene in ΔUvlao2. The same sequence and different sequences are displayed in light blue and white, respectively.
