Fig. 2: Isolation of soil bacteria producing arginoketides and inducing the ors BGC of A. nidulans.
From: Streptomyces polyketides mediate bacteria–fungi interactions across soil environments
a, Schematic depiction of the orsAp-nLuc-GFPs reporter gene fusion (top) and images of the A. nidulans orsAp-nLuc-GFPs reporter strain co-cultured with Streptomyces soil isolates 7, 45, 48, 102, 124, 176, 219 and 280 for 6 h (bottom). The negative control was a A. nidulans orsAp-nLuc-GFPs monoculture and the positive control was A. nidulans orsAp-nLuc-GFPs co-cultured with the known inducer S. iranensis. Scale bar, 500 µm. Images are representative of at least three independent co-cultures. b, Map of Germany with the soil sample origin indicated (top left) and workflow for the isolation of bacteria (right). c, Cultivation of A. nidulans with lydicamycin, linearmycin and soil isolates Streptomyces isolates 219 and 45. Extracted ion chromatograms of orsellinic acid and its derivatives from HPLC–MS analyses of the culture extracts are shown (right). Ion chromatograms for orsellinic acid (m/z 167 [M-H]−), lecanoric acid (m/z 317 [M-H]−) as well as F-9775A and F-9775B (m/z 395 [M-H]−) derived from HPLC–MS analysis of culture extracts. Values depicted on the y axes indicate peak intensities.
