Extended Data Fig. 9: Quantification of LePin signal on the isolated free algae in Xenia by FACS. | Nature Microbiology

Extended Data Fig. 9: Quantification of LePin signal on the isolated free algae in Xenia by FACS.

From: Coral–algal endosymbiosis characterized using RNAi and single-cell RNA-seq

Extended Data Fig. 9: Quantification of LePin signal on the isolated free algae in Xenia by FACS.

a, b, Gating strategy for the free algae in Xenia. The algae are first gated based on the DAPI staining of the nuclei and algae autofluoresce (Cy5.5 signal) (a, free algae gate1). The algae are further gated based on the forward scatter (FSC) and side scatter (SSC) signals to further exclude those algae that are inside the Xenia cells (b, free algae gate2). c-e, LePin signal distribution on free algae in control (c) and LePin knocking down samples (d,e). Color codes for individual animals. The percentage of algae with high LePin signals (as indicated by the brackets) are quantified and labeled.

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