Fig. 1: \({\mathbf{SO}}_{\mathbf{4}}^{{\mathbf{2-}}}\) growth dependency of M. thermolithotrophicus.
From: Assimilatory sulfate reduction in the marine methanogen Methanothermococcus thermolithotrophicus
a, M. thermolithotrophicus cultures grown on Na2S (black squares, 0.5 mM) and Na2SO4 (grey squares, 0.5 mM). The consumption or release of \({{\rm{SO}}}_{4}^{2-}\) in Na2S or Na2SO4 cultures are shown by black and grey triangles, respectively. Data are presented as mean ± s.d. and individual values are shown as spheres (n = 3 replicates). Differences between expected (0.5 mM) and measured (0.13 mM) \({{\rm{SO}}}_{4}^{2-}\) concentration for the initial point are considered to be due to an artefact from the medium (see Methods). b, M. thermolithotrophicus grown on 10 mM Na2SO4 in three independent fermenters. The sampling points are represented by grey squares. c, Molybdate (Na2MoO4) inhibition of Na2SO4 assimilatory and dissimilatory archaea. Growth experiments for M. thermolithotrophicus were performed in duplicates and for A. fulgidus in quadruplicates (left) or triplicates (right). Data are represented as mean and for the triplicates and quadruplicates ± s.d. d, Predicted operon for \({{\rm{SO}}}_{4}^{2-}\) reduction from the whole-genome shotgun sequence of M. thermolithotrophicus.
