Extended Data Fig. 5: MtAPSK belongs to the APSK family.
From: Assimilatory sulfate reduction in the marine methanogen Methanothermococcus thermolithotrophicus
a, Left panel, homodimeric MtAPSK apo (orange) superposed to its closest homologue Synechocystis sp. PCC 6803 (SsAPSK, white, PDB: 5CB6) in complex with APS and AMP-PnP. The ligands are shown in sticks and spheres and the missing part 125–152 in MtAPSK (indicated by balls) is highlighted in black in SsAPSK. Carbon, nitrogen, oxygen, sulfur and phosphorus are coloured in light orange/white/cyan, blue, red, yellow and orange, respectively. Right panel: superposition of MtAPSK (wheat), AtAPSK (orange, PDB: 3UIE), ApAPSK (slate, PDB: 2YVU) and PcAPSK (white, PDB: 1M7H) on one monomer and shown in cartoon. The active site position is indicated by a black arrow. Abbreviations and rmsd can be found in Supplementary Table 2. b, c, Catalytic site of apo MtAPSK (b) and SsAPSK bound to APS and AMP-PnP (c). Elements are coloured as in (a), left panel. In (b), arrows indicate the missing part 125–152.
