Extended Data Fig. 2: Impact of Molybdate (\({\mathbf{MoO}}_{\mathbf{4}}^{{\mathbf{2-}}}\)) on \({\mathbf{SO}}_{\mathbf{4}}^{{\mathbf{2-}}}\)-reducing archaea.
From: Assimilatory sulfate reduction in the marine methanogen Methanothermococcus thermolithotrophicus
a, \({{\rm{MoO}}}_{4}^{2-}\) tolerance of M. thermolithotrophicus. The archaeon was grown on \({{\rm{SO}}}_{4}^{2-}\) (grey square), \({{\rm{SO}}}_{4}^{2-}\) supplemented with an equimolar amount of \({{\rm{MoO}}}_{4}^{2-}\) (wheat square) and \({{\rm{SO}}}_{4}^{2-}\) supplemented with an excess of \({{\rm{MoO}}}_{4}^{2-}\) (dark red square). As a control, Na2S grown cultures (S2-, black triangle) and Na2S grown cultures with an excess of \({{\rm{MoO}}}_{4}^{2-}\) (red triangle) were used. This growth experiment was performed in duplicates. b, Effect of \({{\rm{MoO}}}_{4}^{2-}\):\({{\rm{SO}}}_{4}^{2-}\) ratios in M. thermolithotrophicus cultures grown on 0.5 mM Na2SO4. Grey squares indicate the growth curve of \({{\rm{SO}}}_{4}^{2-}\)-reducers without addition of \({{\rm{MoO}}}_{4}^{2-}\). The red squares indicate the growth curve of \({{\rm{SO}}}_{4}^{2-}\) reducers exposed to 5 mM of \({{\rm{MoO}}}_{4}^{2-}\), followed by the addition of 25 mM Na2SO4. Black, dashed arrows indicate time of \({{\rm{MoO}}}_{4}^{2-}\) and \({{\rm{SO}}}_{4}^{2-}\) addition. This growth experiment was performed in triplicates. c, Archaeoglobus fulgidus sensitivity towards \({{\rm{MoO}}}_{4}^{2-}\). Here, a \({{\rm{MoO}}}_{4}^{2-}\):\({{\rm{SO}}}_{4}^{2-}\) ratio of 0.001:1 is sufficient to inhibit growth of A. fulgidus. The data shown are quadruplicates except for the lowest and the highest \({{\rm{MoO}}}_{4}^{2-}\):\({{\rm{SO}}}_{4}^{2-}\) ratio, which were performed in triplicates. All experiments are represented as data mean and for b, c ± standard deviation (s.d.).
