Extended Data Fig. 4: A significant proportion of A. muciniphila genes required for growth in mucin medium are specific to Akkermansia/Verrucomicrobia.
(a) Number of genes required for optimal A. muciniphila growth in mucin medium that lack functional annotations. Genes corresponding to Tn mutants with a Log2 > 2 fold decrease in abundance in mucin medium were used as the query for a BLAST search to identify potential homologs. The plot represents the number of genes encoding hypothetical proteins that were unique to Akkermansia spp. (Akk), homologs in other members of the PVC super phylum (PVC), homologs in other bacteria (other), and genes annotated as conserved hypothetical proteins (conserved). (b) Distribution of genes with Pfam designations belonging to pili or type II secretion families (Pili/T2SS), or TPR families in the INSeq analysis of genes required for growth in mucin medium in vitro, (c) in the cecum of germ-free mice, and (d) in the cecum of conventional mice.
