Extended Data Fig. 5: Evidence for the presence of a stable Mul1A-Mul1B protein complex. | Nature Microbiology

Extended Data Fig. 5: Evidence for the presence of a stable Mul1A-Mul1B protein complex.

From: A genetic system for Akkermansia muciniphila reveals a role for mucin foraging in gut colonization and host sterol biosynthesis gene expression

Extended Data Fig. 5

Transcriptional analysis of Mul1 operons. (a) View of RNA-seq reads generated from wild type A. muciniphila grown in mucin medium mapped to genes in the mul1 and mul2 loci. (b) Growth curves for wild type A. muciniphila and mutants in mul1B and mul2B grown in triplicate in synthetic medium or with mucin as the sole carbon and nitrogen source and corresponding microscopy with FL-mucin (green). Cells are stained with anti-Akkermansia antisera (white). The scale bar is 1 μm. (c) Coomassie blue stained SDS-PAGE gel showing eluted proteins following immunoprecipitation with anti-Mul1 antibodies. Immunoprecipitations were performed with cell lysates from wild type A. muciniphila and in mul1A mutants. (d) Depiction of Conserved Domains (colours) in Muc5AC and locations of peptides identified as co-precipitating with Mul1A (vertical bars). The experiment was performed in triplicate.

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