Extended Data Fig. 9: Characterization of PurZ0 enzymes of different origins.
From: Alternative Z-genome biosynthesis pathway shows evolutionary progression from Archaea to phage
a-d, Gel analysis of the purified enzymes. 4–20% SDS gel with lane 1, molecular weight marker; and lanes 2, 3 and 4 with 1, 2, 4 μg of purified SpPurZ0, MptPurZ0, MpsPurZ0, MsPurZ0 respectively. e–h, Spectrometric assays of PurZ0 enzymes as indicated. i-l, PurZ0-catalyzed phosphate release detected by the phosphomolybdate assay; (+): the complete assay with GTP-dGMP, Asp and PurZ0 (-): without PurZ0, Asp (-): without Asp-Na+ as controls.
