Extended Data Fig. 4: Chemotactic ability of V. cholerae vc1313 mutant towards Arg.
From: d-amino acids signal a stress-dependent run-away response in Vibrio cholerae
a, Comparison between D-Arg and D-Lys chemotaxis responses in V. cholerae. b, Chemotaxis response to L- and D-Arg increasing concentrations for V. cholerae ΔbsrV and ΔbsrV Δvc1313 double mutant. Significant differences (unpaired t-test) are indicated by *** (p < 0.001), n = 3. c, Chemotactic bias of V. cholerae ΔbsrV and ΔbsrV Δvc1313, measured in microfluidics devices, towards a reservoir containing either 500 µM D-Arg in motility medium or only motility medium (blank control), 1, 2 and 3 h after the preparation of the device. d, Difference between the chemotactic bias (that is, the ratio vch/v0 of the chemotactic drift velocity vch over the cell swimming speed v0, see Methods) towards D-Arg and the corresponding control of the experiments of (b) as a function of the time since the preparation of the microfluidic device. e, Population averaged swimming speed measured in the experiments of (b, c). Each data point of in c, d and e represents the mean and error bars ± SEM of n = 6 biologically independent replicates. Significant differences (unpaired t-test) are indicated by ns (not-significant), * (p < 0.05) ** (p < 0.01) or *** (p < 0.001). See source data.
